Hotspot

trnS-GCU-trnG-UCC

LSC · 8540-9605 · 1066 bp

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Pi 0.0316
MarkerSeek score 73.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1066
variable_sites
49
pi
0.0316
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CTTTAGCCCACTCAGCCATC ACGAATCACACTTTTACCACT 55.0 38.1 58.3 55.5 1125-1147 bp 1.0 55.2
trnS-GCU-trnG-UCC_p2 TAGCAATCCGACGCTTTAGC ACGAATCACACTTTTACCACT 50.0 38.1 58.4 55.5 1138-1160 bp 1.0 54.3
trnS-GCU-trnG-UCC_p3 CTTTAGCCCACTCAGCCATC AGAACGAATCACACTTTTACCAC 55.0 39.1 58.3 57.3 1128-1150 bp 1.0 54.2
trnS-GCU-trnG-UCC_p4 CTTTAGCCCACTCAGCCATC GAACGAATCACACTTTTACCACT 55.0 39.1 58.3 57.3 1127-1149 bp 1.0 54.2
trnS-GCU-trnG-UCC_p5 TAGCAATCCGACGCTTTAGC GAACGAATCACACTTTTACCACT 50.0 39.1 58.4 57.3 1140-1162 bp 1.0 53.3