Hotspot

matK-rps16

LSC · 3377-5021 · 1645 bp

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Pi 0.0183
MarkerSeek score 62.10
Primer available yes
Label matK-rps16

Feature Metadata

label_name
matK-rps16
feature_type
igs
region
LSC
length_bp
1645
variable_sites
44
pi
0.0183
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-rps16_p1 TCCTTGTAATTCTGCCATTT GTGCTCAACCCACAGGAAC 35.0 57.9 52.7 58.7 1761-1768 bp 1.0 44.3
matK-rps16_p2 TCCTTGTAATTCTGCCATTT TGCTCAACCCACAGGAACT 35.0 52.6 52.7 58.8 1760-1767 bp 1.0 44.3
matK-rps16_p3 ATCCTTGTAATTCTGCCATT GTGCTCAACCCACAGGAAC 35.0 57.9 52.5 58.7 1762-1769 bp 1.0 44.3
matK-rps16_p4 ATCCTTGTAATTCTGCCATTT GTGCTCAACCCACAGGAAC 33.3 57.9 53.4 58.7 1762-1769 bp 1.0 44.3
matK-rps16_p5 ATCCTTGTAATTCTGCCATT TGCTCAACCCACAGGAACT 35.0 52.6 52.5 58.8 1761-1768 bp 1.0 44.3