Hotspot

rps16

LSC · 5420-6566 · 1147 bp

Back to Taihangia

Pi 0.00262

MarkerSeek score 58.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1147
variable_sites: 3
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGGAACTGGCAAAGAAATGA	TGTAACGACCGAACCAATGA	40.0	45.0	55.1	57.2	1328-1329 bp	1.0	50.8
rps16_p2	ACTGGCAAAGAAATGAAAAGGT	TGTAACGACCGAACCAATGA	36.4	45.0	57.0	57.2	1324-1325 bp	1.0	49.5
rps16_p3	AAGGAACTGGCAAAGAAATGA	TGTAACGACCGAACCAATGA	38.1	45.0	56.0	57.2	1329-1330 bp	1.0	49.4
rps16_p4	AGGAACTGGCAAAGAAATGAA	TGTAACGACCGAACCAATGA	38.1	45.0	56.0	57.2	1328-1329 bp	1.0	49.4
rps16_p5	AGGAACTGGCAAAGAAATGAAA	TGTAACGACCGAACCAATGA	36.4	45.0	56.7	57.2	1328-1329 bp	1.0	47.4