Hotspot

rpl22

LSC · 84984-85412 · 429 bp

Back to Taihangia

Pi 0.00466

MarkerSeek score 47.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 429
variable_sites: 2
pi: 0.00466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	ACCGAAAAACGTCATATCCGT	TCGTTTATTAATGAGAGGTGAACC	42.9	37.5	58.0	56.8	561 bp	1.0	40.3
rpl22_p2	CCGAAAAACGTCATATCCGTG	TCGTTTATTAATGAGAGGTGAACC	47.6	37.5	57.7	56.8	560 bp	1.0	40.3
rpl22_p3	ACCGAAAAACGTCATATCCGT	CGTTTATTAATGAGAGGTGAACC	42.9	39.1	58.0	55.4	560 bp	1.0	40.3
rpl22_p4	ACCGAAAAACGTCATATCCGT	ATCGTTTATTAATGAGAGGTGAACC	42.9	36.0	58.0	57.2	562 bp	1.0	40.3
rpl22_p5	CCGAAAAACGTCATATCCGTG	CGTTTATTAATGAGAGGTGAACC	47.6	39.1	57.7	55.4	559 bp	1.0	40.3