Hotspot

psbD

LSC · 33692-34753 · 1062 bp

Back to Taihangia

Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	AGAAGGGGCAATGTAGGAGA	CCCCCAGTAGTTTACCGGAT	50.0	55.0	57.7	58.5	1277 bp	1.0	73.9
psbD_p2	TTCGAAAGAAGGGGCAATGT	CCCCCAGTAGTTTACCGGAT	45.0	55.0	57.7	58.5	1283 bp	1.0	73.8
psbD_p3	AGAAGGGGCAATGTAGGAGA	AACATGAGCCCCCAGTAGTT	50.0	50.0	57.7	58.6	1285 bp	1.0	72.9
psbD_p4	AGAAGGGGCAATGTAGGAGA	ACATGAGCCCCCAGTAGTTT	50.0	50.0	57.7	58.6	1284 bp	1.0	72.9
psbD_p5	TTCGAAAGAAGGGGCAATGT	ACATGAGCCCCCAGTAGTTT	45.0	50.0	57.7	58.6	1290 bp	1.0	72.8