Hotspot

ndhF

SSC · 111459-113717 · 2259 bp

Back to Taihangia

Pi 0.00177

MarkerSeek score 43.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 4
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTTCCGGGACTAAACAGGA	ACCAAGCCTAACTCTTCACC	50.0	50.0	58.0	57.1	2855-2868 bp	1.0	73.8
ndhF_p2	GCAGAATACCGTCACCCATT	ACCAAGCCTAACTCTTCACC	50.0	50.0	58.0	57.1	2935-2948 bp	1.0	73.6
ndhF_p3	ACAGGAACAAGAGGGATCCA	ACCAAGCCTAACTCTTCACC	50.0	50.0	58.0	57.1	2841-2854 bp	1.0	73.6
ndhF_p4	AAGAGGGATCCACCGAAGAA	ACCAAGCCTAACTCTTCACC	50.0	50.0	58.0	57.1	2833-2846 bp	1.0	73.6
ndhF_p5	GAAGATCCTTCCCCTTCCCT	ACCAAGCCTAACTCTTCACC	55.0	50.0	58.1	57.1	2816-2829 bp	1.0	73.2