Hotspot

accD

LSC · 58164-59705 · 1542 bp

Back to Taihangia

Pi 0.0013

MarkerSeek score 59.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1542
variable_sites: 2
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AGGAGAAATAGCCTTGCGTT	TCTTCGTTATTTTCTTTTTCGT	45.0	27.3	57.2	52.7	1713 bp	1.0	40.1
accD_p2	TAGGAGAAATAGCCTTGCGT	TCTTCGTTATTTTCTTTTTCGT	45.0	27.3	56.3	52.7	1714 bp	1.0	40.1
accD_p3	AGGAGAAATAGCCTTGCGTT	TCTTCGTTATTTTCTTTTTCGTATCT	45.0	26.9	57.2	55.8	1713 bp	1.0	40.1
accD_p4	AGGAGAAATAGCCTTGCGTT	TCTTCGTTATTTTCTTTTTCGTA	45.0	26.1	57.2	52.8	1713 bp	1.0	40.1
accD_p5	AGGAGAAATAGCCTTGCGTTA	TCTTCGTTATTTTCTTTTTCGT	42.9	27.3	57.1	52.7	1713 bp	1.0	40.1