Hotspot

ndhF-rpl32

SSC · 113718-114793 · 1076 bp

Back to Taihangia

Pi 0.00372

MarkerSeek score 60.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1076
variable_sites: 4
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGACGAAGATTTCTGGTTGC	AAAAAGCTTTCAGTGCTGCC	50.0	45.0	57.5	57.8	1286 bp	1.0	74.3
ndhF-rpl32_p2	ACGAAGATTTCTGGTTGCCT	AAAAAGCTTTCAGTGCTGCC	45.0	45.0	57.4	57.8	1284 bp	1.0	73.9
ndhF-rpl32_p3	CGACGAAGATTTCTGGTTGC	TTTCAGTGCTGCCCAATACC	50.0	50.0	57.5	58.5	1279 bp	1.0	72.5
ndhF-rpl32_p4	ACGAAGATTTCTGGTTGCCT	TTTCAGTGCTGCCCAATACC	45.0	50.0	57.4	58.5	1277 bp	1.0	72.1
ndhF-rpl32_p5	CGACGAAGATTTCTGGTTGC	CTGCCCAATACCCTTTCCTT	50.0	50.0	57.5	57.5	1271 bp	1.0	71.9