Hotspot

ycf1-ndhF

SSC · 111412-111458 · 47 bp

Back to Taihangia

Pi 0.0606

MarkerSeek score 63.60

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 47
variable_sites: 2
pi: 0.0606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACTTCCGGGACTAAACAGGA	GAGGGTATCAAATCTGCGGG	50.0	55.0	58.0	58.1	508-521 bp	1.0	83.1
ycf1-ndhF_p2	ACTTCCGGGACTAAACAGGA	TGGGGTCGTAGGAATTCCTT	50.0	50.0	58.0	58.0	968-981 bp	1.0	82.0
ycf1-ndhF_p3	ACAGGAACAAGAGGGATCCA	TGGGGTCGTAGGAATTCCTT	50.0	50.0	58.0	58.0	954-967 bp	1.0	81.8
ycf1-ndhF_p4	AAGAGGGATCCACCGAAGAA	TGGGGTCGTAGGAATTCCTT	50.0	50.0	58.0	58.0	946-959 bp	1.0	81.8
ycf1-ndhF_p5	GCAGAATACCGTCACCCATT	TGGGGTCGTAGGAATTCCTT	50.0	50.0	58.0	58.0	1048-1061 bp	1.0	81.7