Hotspot

trnA-UGC-rrn23

IRb · 105524-105686 · 163 bp

Back to Taihangia

Pi 0.0123
MarkerSeek score 59.60
Primer available yes
Label trnA-UGC-rrn23

Feature Metadata

label_name
trnA-UGC-rrn23
feature_type
igs
region
IRb
length_bp
163
variable_sites
2
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC-rrn23_p1 GGTTCGAGTCCGCTTATCTC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 303 bp 1.0 79.4
trnA-UGC-rrn23_p2 GTTCGAGTCCGCTTATCTCC ATTCGGGAATCTCCGGATCT 55.0 50.0 57.9 58.0 302 bp 1.0 79.4
trnA-UGC-rrn23_p3 GGTTCGAGTCCGCTTATCTC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 57.9 57.9 231 bp 1.0 79.0
trnA-UGC-rrn23_p4 GTTCGAGTCCGCTTATCTCC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 57.9 57.9 230 bp 1.0 79.0
trnA-UGC-rrn23_p5 TTCGAGTCCGCTTATCTCCA ATTCGGGAATCTCCGGATCT 50.0 50.0 58.2 58.0 301 bp 1.0 78.5