Hotspot

petA-psbJ

LSC · 63642-64794 · 1153 bp

Back to Taihangia

Pi 0.0139

MarkerSeek score 66.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 16
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCTCTTCTTGGCATCCGTTA	GCCGATACTACTGGAAGGAT	50.0	50.0	58.0	56.2	1365-1367 bp	1.0	66.5
petA-psbJ_p2	TGTTCAAGGCCTTTTGCTCT	GCCGATACTACTGGAAGGAT	45.0	50.0	57.9	56.2	1380-1382 bp	1.0	66.1
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	GCCGATACTACTGGAAGGAT	50.0	50.0	58.1	56.2	1356-1358 bp	1.0	66.1
petA-psbJ_p4	GTGTTCAAGGCCTTTTGCTC	GCCGATACTACTGGAAGGAT	50.0	50.0	57.9	56.2	1381-1383 bp	1.0	65.6
petA-psbJ_p5	CCATTACGTGTTCAAGGCCT	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	1388-1390 bp	1.0	65.3