Hotspot

ndhC-trnV-UAC

LSC · 51792-52458 · 667 bp

Back to Taihangia

Pi 0.006
MarkerSeek score 52.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
667
variable_sites
4
pi
0.006
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGAGACTATCAGAAATGCCCAG AGAAGGTCTACGGTTCGAGT 45.5 50.0 57.6 57.8 751 bp 1.0 59.5
ndhC-trnV-UAC_p2 TGAGACTATCAGAAATGCCCA AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.4 57.8 751 bp 1.0 58.3
ndhC-trnV-UAC_p3 TGAGACTATCAGAAATGCCCAG ACTGCATGTTGGGTCTTTGA 45.5 45.0 57.6 57.6 818 bp 1.0 58.2
ndhC-trnV-UAC_p4 GAGACTATCAGAAATGCCCAGA AGAAGGTCTACGGTTCGAGT 45.5 50.0 57.3 57.8 750 bp 1.0 57.4
ndhC-trnV-UAC_p5 TGAGACTATCAGAAATGCCCA ACTGCATGTTGGGTCTTTGA 42.9 45.0 56.4 57.6 818 bp 1.0 56.9