Hotspot

rrn5-trnR-ACG

IRb · 106980-107239 · 260 bp

Back to Tagetes

Pi 0.0231

MarkerSeek score 65.80

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 260
variable_sites: 6
pi: 0.0231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	344-362 bp	1.0	79.1
rrn5-trnR-ACG_p2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	379-397 bp	1.0	78.5
rrn5-trnR-ACG_p3	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	382-400 bp	1.0	78.5
rrn5-trnR-ACG_p4	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	380-398 bp	1.0	78.5
rrn5-trnR-ACG_p5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	383-401 bp	1.0	78.5