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petA-psbJ

LSC · 62736-63525 · 790 bp

Back to Tagetes

Pi 0.00776

MarkerSeek score 65.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 6
pi: 0.00776
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	949-966 bp	1.0	77.0
petA-psbJ_p2	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	948-965 bp	1.0	77.0
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	50.0	50.0	57.9	57.4	939-956 bp	1.0	76.9
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	GTGATCGGTTTAGTAGGCGT	50.0	50.0	57.9	57.4	938-955 bp	1.0	76.9
petA-psbJ_p5	ACGTGTACAAGGCCTTTTGT	TATTCCGGATTGGGTTCGTC	45.0	50.0	58.0	57.4	912-929 bp	1.0	76.8