Hotspot

trnK-UUU-rps16

LSC · 4341-5061 · 721 bp

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Pi 0.0111
MarkerSeek score 65.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
721
variable_sites
8
pi
0.0111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 918-933 bp 1.0 77.7
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 917-932 bp 1.0 77.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 857-872 bp 1.0 77.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 856-871 bp 1.0 77.1
trnK-UUU-rps16_p5 TCCTAGCCGCACTTAAAAGC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.3 58.3 872-887 bp 1.0 76.3