Hotspot

ycf1

SSC · 111806-117112 · 5307 bp

Back to Taeniophyllum

Pi 0.086

MarkerSeek score 71.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5307
variable_sites: 451
pi: 0.086
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAGGTCTCAATTGCGACTT	TCGGATTTTCGTCGCAATCT	45.0	45.0	57.4	58.0	1448 bp	0.5	55.6
ycf1_p2	ACAGGTCTCAATTGCGACTT	TGATTCTCCCCGAAAACGAC	45.0	50.0	57.4	57.9	771 bp	0.5	54.9
ycf1_p3	ACAGGTCTCAATTGCGACTT	TGGATGGAGAGAGAACACGA	45.0	50.0	57.4	57.8	1262 bp	0.5	53.9
ycf1_p4	ACAGGTCTCAATTGCGACTT	CCCCGAAAACGACCTTTCTT	45.0	50.0	57.4	58.4	764 bp	0.5	52.4
ycf1_p5	ACAGGTCTCAATTGCGACTT	CAAATTCCCGAGTGGAGTGA	45.0	50.0	57.4	57.5	1028 bp	0.5	51.8