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psbE-petL

LSC · 61226-62383 · 1158 bp

Back to Taeniophyllum

Pi 0.0983

MarkerSeek score 83.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 107
pi: 0.0983
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CTTGTCGGCTCTCTGTGAAA	AAGCGCTCTTAGTTCAGCTC	50.0	50.0	57.8	58.0	1914-1939 bp	1.0	87.7
psbE-petL_p2	TCCTTCCAGTAGTCTCGGTC	AAGCGCTCTTAGTTCAGCTC	55.0	50.0	57.9	58.0	2386-2411 bp	1.0	86.5
psbE-petL_p3	CCAACCCAGAATAGGAACCG	AAGCGCTCTTAGTTCAGCTC	55.0	50.0	58.0	58.0	2498 bp	0.5	59.7
psbE-petL_p4	CCAACCCAGAATAGGAACCG	ACCGCGCCTGTATTGTAAAT	55.0	45.0	58.0	58.0	2282 bp	0.5	59.4
psbE-petL_p5	CCAACCCAGAATAGGAACCG	CAAAACCCGATGTCGTAGGT	55.0	50.0	58.0	57.9	2461 bp	0.5	59.0