Hotspot

ycf3-trnS-GGA

LSC · 43275-43888 · 614 bp

Back to Taeniophyllum

Pi 0.0626
MarkerSeek score 84.80
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
614
variable_sites
36
pi
0.0626
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 42.9 45.0 56.6 57.8 678-710 bp 1.0 67.7
ycf3-trnS-GGA_p2 TGGCTACAATTGAAGAGGTCT CGGAGAGAGAGGGATTCGAA 42.9 55.0 56.6 58.3 727-759 bp 1.0 66.5
ycf3-trnS-GGA_p3 TGGCTACAATTGAAGAGGTCTT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 678-710 bp 1.0 65.5
ycf3-trnS-GGA_p4 TTGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 679-711 bp 1.0 65.5
ycf3-trnS-GGA_p5 TGGCTACAATTGAAGAGGTCT AGTTCCAATGCTACGCCTTG 42.9 50.0 56.6 58.5 680-712 bp 1.0 64.9