Hotspot

trnT-GGU-psbD

LSC · 30969-31980 · 1012 bp

Back to Taeniophyllum

Pi 0.0982

MarkerSeek score 88.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 94
pi: 0.0982
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 88.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1121-1147 bp	1.0	87.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1124-1150 bp	1.0	87.7
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.3	57.7	1098-1124 bp	1.0	86.4
trnT-GGU-psbD_p4	TGGTAGAGTAACGCCATGGT	CCCTCCGTAACCAATCATCC	50.0	55.0	58.4	57.7	1120-1146 bp	1.0	85.0
trnT-GGU-psbD_p5	CTCAGTGGTAGAGTAACGCC	CCCTCCGTAACCAATCATCC	55.0	55.0	57.4	57.7	1125-1151 bp	1.0	83.9