Hotspot

rps7

IRa · 128151-128621 · 471 bp

Back to Taeniatherum

Pi 0

MarkerSeek score 33.60

Primer available yes

Label rps7

Feature Metadata

label_name: rps7
feature_type: gene
region: IRa
length_bp: 471
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7_p1	TCTTGAACCTCTTTCACGCT	TCCGATCGAGATGTACGGAT	45.0	50.0	57.1	57.8	546 bp	1.0	71.3
rps7_p2	TCTTTCGAGATCCACCCTACA	TCCGATCGAGATGTACGGAT	47.6	50.0	57.9	57.8	627 bp	1.0	69.8
rps7_p3	TTCTTGAACCTCTTTCACGCT	TCCGATCGAGATGTACGGAT	42.9	50.0	57.8	57.8	547 bp	1.0	69.0
rps7_p4	CTTGAACCTCTTTCACGCTC	TCCGATCGAGATGTACGGAT	50.0	50.0	56.8	57.8	545 bp	1.0	68.6
rps7_p5	TCTTGAACCTCTTTCACGCTC	TCCGATCGAGATGTACGGAT	47.6	50.0	58.2	57.8	546 bp	1.0	68.6