Hotspot

ndhH

SSC · 114301-115482 · 1182 bp

Back to Taeniatherum

Pi 0.000846

MarkerSeek score 59.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 1
pi: 0.000846
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGGAGGGTCAAAATGGGTAGA	TTTGATGAGCCTCGCTTTGA	47.6	45.0	58.1	57.8	1364 bp	1.0	70.0
ndhH_p2	AAAGGAGGGTCAAAATGGGT	TTTGATGAGCCTCGCTTTGA	45.0	45.0	56.9	57.8	1366 bp	1.0	69.8
ndhH_p3	TCCCAAAAGGAGGGTCAAAA	TTTGATGAGCCTCGCTTTGA	45.0	45.0	56.8	57.8	1371 bp	1.0	69.3
ndhH_p4	AGGAGGGTCAAAATGGGTAGA	TTGATGAGCCTCGCTTTGAG	47.6	50.0	58.1	58.3	1363 bp	1.0	69.3
ndhH_p5	AAAGGAGGGTCAAAATGGGT	TTGATGAGCCTCGCTTTGAG	45.0	50.0	56.9	58.3	1365 bp	1.0	69.1