Hotspot

rpl32-trnL-UAG

SSC · 105900-106609 · 710 bp

Back to Taeniatherum

Pi 0.00141

MarkerSeek score 59.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 710
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAGCGGTAATGAGCATCCAA	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	845-846 bp	1.0	79.8
rpl32-trnL-UAG_p2	TTTTCGAGCGGTAATGAGCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	57.9	58.0	850-851 bp	1.0	79.5
rpl32-trnL-UAG_p3	GAGCGGTAATGAGCATCCAA	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	848-849 bp	1.0	79.3
rpl32-trnL-UAG_p4	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	820-821 bp	1.0	79.1
rpl32-trnL-UAG_p5	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	819-820 bp	1.0	79.1