Hotspot

ndhB-rps7

IRb · 88890-89188 · 299 bp

Back to Taeniatherum

Pi 0.00669

MarkerSeek score 56.30

Primer available yes

Label ndhB-rps7

Feature Metadata

label_name: ndhB-rps7
feature_type: igs
region: IRb
length_bp: 299
variable_sites: 2
pi: 0.00669
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-rps7_p1	AGAGAAGCAAATGAAACGCT	ATGGCAGAGGCAAATAGAGC	40.0	50.0	56.0	58.0	417 bp	1.0	63.9
ndhB-rps7_p2	TGAAGAGAAGCAAATGAAACGC	ATGGCAGAGGCAAATAGAGC	40.9	50.0	58.1	58.0	420 bp	1.0	63.3
ndhB-rps7_p3	GAAGAGAAGCAAATGAAACGCT	ATGGCAGAGGCAAATAGAGC	40.9	50.0	57.8	58.0	419 bp	1.0	63.0
ndhB-rps7_p4	AAGAGAAGCAAATGAAACGCT	ATGGCAGAGGCAAATAGAGC	38.1	50.0	56.7	58.0	418 bp	1.0	62.0
ndhB-rps7_p5	AGAGAAGCAAATGAAACGCT	GTAGATGCTGCCAAAGGGAG	40.0	55.0	56.0	58.3	474 bp	1.0	61.4