Hotspot

infA-rps8

LSC · 77013-77092 · 80 bp

Back to Taeniatherum

Pi 0.0125

MarkerSeek score 59.70

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 80
variable_sites: 1
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AACATTCCATTGGGTAGGGC	GGGTGGAATGGGAATTGCTA	50.0	50.0	57.8	57.5	284 bp	1.0	75.4
infA-rps8_p2	GGGTAGGGCTTCCGTAACTA	GGGTGGAATGGGAATTGCTA	55.0	50.0	58.2	57.5	273 bp	1.0	74.9
infA-rps8_p3	ATTGGGTAGGGCTTCCGTAA	GGGTGGAATGGGAATTGCTA	50.0	50.0	58.4	57.5	276 bp	1.0	73.4
infA-rps8_p4	AACATTCCATTGGGTAGGGC	TTGGGTGGAATGGGAATTGC	50.0	50.0	57.8	58.7	286 bp	1.0	73.2
infA-rps8_p5	CATTCCATTGGGTAGGGCTT	GGGTGGAATGGGAATTGCTA	50.0	50.0	57.5	57.5	282 bp	1.0	73.0