Hotspot

petA-psbJ

LSC · 61180-62005 · 826 bp

Back to Taeniatherum

Pi 0

MarkerSeek score 59.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTGCTGTGATTGG	50.0	50.0	58.0	57.1	1015-1020 bp	1.0	73.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTGCTGTGATTGG	50.0	50.0	57.9	57.1	998-1003 bp	1.0	73.1
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTGCTGTGATTGG	50.0	50.0	58.2	57.1	1012-1017 bp	1.0	72.1
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	ACTGTAGCTGGTATTGCTGTG	50.0	47.6	58.0	58.0	1021-1026 bp	1.0	71.9
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	ACTGTAGCTGGTATTGCTGTG	50.0	47.6	57.9	58.0	1004-1009 bp	1.0	71.8