Hotspot

rbcL-psaI

LSC · 56147-57814 · 1668 bp

Back to Taeniatherum

Pi 0.0012

MarkerSeek score 59.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1668
variable_sites: 2
pi: 0.0012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGCAGCTTGTGAAGTATGGA	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.3	57.9	1803 bp	1.0	77.3
rbcL-psaI_p2	AGTTCGAGCCGGTAGATACT	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.7	57.9	1768 bp	1.0	76.7
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.6	57.9	1782 bp	1.0	76.6
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	GTCATTGCAATTGCCGGAAA	45.0	45.0	57.6	57.9	1787 bp	1.0	76.3
rbcL-psaI_p5	AGCTTGCAAATGGAGTCCTG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.5	57.9	1830 bp	1.0	75.8