Hotspot

ycf1

Genome · 111173-113360 · 2188 bp

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Pi 0.462

MarkerSeek score 79.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 2188
variable_sites: 879
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGAACAATAGAAGTCGGCC	GCCATAGGTTTTCCCCGATT	50.0	50.0	58.0	57.9	2996 bp	0.5	59.4
ycf1_p2	TAGAAGTCGGCCTAACCCAA	GCCATAGGTTTTCCCCGATT	50.0	50.0	58.1	57.9	2988 bp	0.5	59.0
ycf1_p3	GAAGTCGGCCTAACCCAAAA	GCCATAGGTTTTCCCCGATT	50.0	50.0	58.1	57.9	2986 bp	0.5	58.7
ycf1_p4	ATAGAAGTCGGCCTAACCCA	GCCATAGGTTTTCCCCGATT	50.0	50.0	57.8	57.9	2989 bp	0.5	58.1
ycf1_p5	ACGAACAATAGAAGTCGGCC	ATTGGACGCTAAGACAAGGC	50.0	50.0	58.0	58.3	2407 bp	0.5	57.9