Hotspot

ndhH

Genome · 114018-115199 · 1182 bp

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Pi 0.479

MarkerSeek score 81.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1182
variable_sites: 501
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCCTTGTCTTAGCGTCCAAT	CGTTGTTGTATACCTGCGGA	50.0	50.0	58.3	58.0	1969 bp	0.5	57.9
ndhH_p2	GCCTTGTCTTAGCGTCCAAT	TGACTGCTATAGAGGGTCCG	50.0	55.0	58.3	58.0	2106 bp	0.5	57.8
ndhH_p3	GCCTTGTCTTAGCGTCCAAT	TGAAGAATTCCCAAAGGGCC	50.0	50.0	58.3	58.1	2008 bp	0.5	57.4
ndhH_p4	GCCTTGTCTTAGCGTCCAAT	CCGTCTGCTAGAGCTTGAAG	50.0	55.0	58.3	58.1	2023 bp	0.5	57.3
ndhH_p5	GGGGGTCCGTTGAATTTCAA	CGTTGTTGTATACCTGCGGA	50.0	50.0	58.4	58.0	1697 bp	0.5	57.0