Hotspot

ndhE

Genome · 119186-119491 · 306 bp

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Pi 0.46

MarkerSeek score 72.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: Genome
length_bp: 306
variable_sites: 110
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GATGGGGCTATTTCGTTGGT	GCCTTACATCCATCCCAAGG	50.0	55.0	57.9	58.0	1234 bp	0.5	59.5
ndhE_p2	GATGGGGCTATTTCGTTGGT	AACCTGCAAATAGGGGCAAA	50.0	45.0	57.9	58.0	1550 bp	0.5	59.4
ndhE_p3	GATGGGGCTATTTCGTTGGT	ACCTGCAAATAGGGGCAAAA	50.0	45.0	57.9	58.0	1549 bp	0.5	59.4
ndhE_p4	GGGGTCTCGGAGTGGTATTA	GCCTTACATCCATCCCAAGG	55.0	55.0	57.9	58.0	1458 bp	0.5	59.3
ndhE_p5	GGGGTCTCGGAGTGGTATTA	ACCTGCAAATAGGGGCAAAA	55.0	45.0	57.9	58.0	1773 bp	0.5	59.2