Hotspot

ndhD

Genome · 120186-121688 · 1503 bp

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Pi 0.483

MarkerSeek score 80.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1503
variable_sites: 612
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CCTTGGGATGGATGTAAGGC	GGACAAGGCCTAAGCAAGTT	55.0	50.0	58.0	58.1	1971 bp	0.5	59.2
ndhD_p2	CCTTGGGATGGATGTAAGGC	AAAGCACTTCTCCCCTTTCG	55.0	50.0	58.0	58.1	2374 bp	0.5	59.1
ndhD_p3	CCTTGGGATGGATGTAAGGC	ATTCGGTCTGGCCTTTCTTC	55.0	50.0	58.0	57.9	1882 bp	0.5	59.0
ndhD_p4	TACGAGCTTGTCCTACGGAT	GGACAAGGCCTAAGCAAGTT	50.0	50.0	58.0	58.1	2006 bp	0.5	58.9
ndhD_p5	TACGAGCTTGTCCTACGGAT	AAAGCACTTCTCCCCTTTCG	50.0	50.0	58.0	58.1	2409 bp	0.5	58.8