Hotspot

ccsA

Genome · 121908-122888 · 981 bp

Back to Tacinga

Pi 0.474

MarkerSeek score 77.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 981
variable_sites: 341
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCATGAGTGGTTTCATTGC	GGGATATTGGGCAGCGTTAA	50.0	50.0	58.0	58.0	1931 bp	0.5	59.8
ccsA_p2	GTTTACCCGGATTTCGCTCT	GGGATATTGGGCAGCGTTAA	50.0	50.0	58.0	58.0	1670 bp	0.5	59.6
ccsA_p3	GGCATGAGTGGTTTCATTGC	AACTCCTCCAATCTGGACGA	50.0	50.0	58.0	58.1	1786 bp	0.5	59.5
ccsA_p4	GTATTGGCGTTTACCCGGAT	GGGATATTGGGCAGCGTTAA	50.0	50.0	58.0	58.0	1678 bp	0.5	59.5
ccsA_p5	GGCATGAGTGGTTTCATTGC	CTTAGGAAGCAGTGCGAGAG	50.0	55.0	58.0	58.1	1700 bp	0.5	59.4