Hotspot

trnL-UAG-rpl32

Genome · 123085-123193 · 109 bp

Back to Tacinga

Pi 0.417

MarkerSeek score 72.70

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: Genome
length_bp: 109
variable_sites: 35
pi: 0.417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	ATCCGCATAAAAGAGCTGCA	GGGATATTGGGCAGCGTTAA	45.0	50.0	58.0	58.0	855 bp	0.5	59.5
trnL-UAG-rpl32_p2	TCCGCATAAAAGAGCTGCAT	GGGATATTGGGCAGCGTTAA	45.0	50.0	58.0	58.0	854 bp	0.5	59.5
trnL-UAG-rpl32_p3	CTCGCACTGCTTCCTAAGAG	GGGATATTGGGCAGCGTTAA	55.0	50.0	58.1	58.0	249 bp	0.5	59.2
trnL-UAG-rpl32_p4	CTCTCGCACTGCTTCCTAAG	GGGATATTGGGCAGCGTTAA	55.0	50.0	58.1	58.0	251 bp	0.5	59.2
trnL-UAG-rpl32_p5	TCCGTTAAGAGTCCCGAAGT	GGGATATTGGGCAGCGTTAA	50.0	50.0	58.1	58.0	754 bp	0.5	59.1