Hotspot

ndhG-ndhE

Genome · 118934-119185 · 252 bp

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Pi 0.498

MarkerSeek score 75.40

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: Genome
length_bp: 252
variable_sites: 110
pi: 0.498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	GATGGGGCTATTTCGTTGGT	GCCTTACATCCATCCCAAGG	50.0	55.0	57.9	58.0	1234 bp	0.5	59.5
ndhG-ndhE_p2	GATGGGGCTATTTCGTTGGT	AGTCCAATAGCAGCTTCAGC	50.0	50.0	57.9	58.0	728 bp	0.5	59.3
ndhG-ndhE_p3	GGGGTCTCGGAGTGGTATTA	GCCTTACATCCATCCCAAGG	55.0	55.0	57.9	58.0	1458 bp	0.5	59.3
ndhG-ndhE_p4	GGGGTCTCGGAGTGGTATTA	AGTCCAATAGCAGCTTCAGC	55.0	50.0	57.9	58.0	952 bp	0.5	59.1
ndhG-ndhE_p5	TCTTTCTGGGGTCAGGTCTT	GCCTTACATCCATCCCAAGG	50.0	55.0	57.9	58.0	1485 bp	0.5	58.9