Hotspot

ndhI-ndhG

Genome · 118036-118402 · 367 bp

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Pi 0.457

MarkerSeek score 75.40

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: Genome
length_bp: 367
variable_sites: 139
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TGATCACATCGGAGCGTTTT	ACCAACGAAATAGCCCCATC	45.0	50.0	57.9	57.9	1052 bp	0.5	58.8
ndhI-ndhG_p2	TTGATCACATCGGAGCGTTT	ACCAACGAAATAGCCCCATC	45.0	50.0	57.9	57.9	1053 bp	0.5	58.8
ndhI-ndhG_p3	TGATCACATCGGAGCGTTTT	TAATACCACTCCGAGACCCC	45.0	55.0	57.9	57.9	828 bp	0.5	58.6
ndhI-ndhG_p4	TTGATCACATCGGAGCGTTT	TAATACCACTCCGAGACCCC	45.0	55.0	57.9	57.9	829 bp	0.5	58.6
ndhI-ndhG_p5	TGATCACATCGGAGCGTTTT	AAGACCTGACCCCAGAAAGA	45.0	50.0	57.9	57.9	801 bp	0.5	58.3