Hotspot

ndhA-ndhI

Genome · 117388-117519 · 132 bp

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Pi 0.419

MarkerSeek score 77.10

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: Genome
length_bp: 132
variable_sites: 52
pi: 0.419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	TGGGTGGTTGGAATCTCTCT	CGTACCGCTTGTTGACCATA	50.0	50.0	58.0	58.0	448 bp	0.5	59.6
ndhA-ndhI_p2	TGGGTGGTTGGAATCTCTCT	AAAACGCTCCGATGTGATCA	50.0	45.0	58.0	57.9	557 bp	0.5	58.9
ndhA-ndhI_p3	TGGGTGGTTGGAATCTCTCT	AAACGCTCCGATGTGATCAA	50.0	45.0	58.0	57.9	556 bp	0.5	58.9
ndhA-ndhI_p4	TGGGTGGTTGGAATCTCTCT	GGTAAACGACCCAGAGCAAT	50.0	50.0	58.0	57.9	835 bp	0.5	58.8
ndhA-ndhI_p5	TGCGAGAGATTGCCTTTTGA	CGTACCGCTTGTTGACCATA	45.0	50.0	57.8	58.0	599 bp	0.5	58.5