Hotspot

rpl32-ycf1

Genome · 110994-111172 · 179 bp

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Pi 0.41

MarkerSeek score 73.10

Primer available yes

Label rpl32-ycf1

Feature Metadata

label_name: rpl32-ycf1
feature_type: igs
region: Genome
length_bp: 179
variable_sites: 73
pi: 0.41
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ycf1_p1	AGTAAGGGGGCAATACCTGT	GCCTACTCAATACTAGGCGC	50.0	55.0	58.0	57.9	1054 bp	0.5	59.3
rpl32-ycf1_p2	GGGATATTGGGCAGCGTTAA	GCCTACTCAATACTAGGCGC	50.0	55.0	58.0	57.9	544 bp	0.5	59.3
rpl32-ycf1_p3	AGTAAGGGGGCAATACCTGT	AAAACCAGTTGATGCGGCTA	50.0	45.0	58.0	58.1	987 bp	0.5	59.1
rpl32-ycf1_p4	AGTAAGGGGGCAATACCTGT	TAAAACCAGTTGATGCGGCT	50.0	45.0	58.0	58.1	988 bp	0.5	59.1
rpl32-ycf1_p5	GGGATATTGGGCAGCGTTAA	AAAACCAGTTGATGCGGCTA	50.0	45.0	58.0	58.1	477 bp	0.5	59.0