Hotspot

ndhI

SSC · 126869-127411 · 543 bp

Back to Tacca

Pi 0.0203
MarkerSeek score 54.40
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
543
variable_sites
11
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 ATCCCCGATGGTCCAAAAAC TGTAACCATAGAGGAGCCGT 50.0 50.0 58.2 57.8 1884 bp 1.0 81.5
ndhI_p2 CATCCCCGATGGTCCAAAAA TGTAACCATAGAGGAGCCGT 50.0 50.0 58.2 57.8 1885 bp 1.0 81.5
ndhI_p3 CAGCGAAGTGATACCATCCC TGTAACCATAGAGGAGCCGT 55.0 50.0 58.1 57.8 1899 bp 0.5 57.7
ndhI_p4 CCAGCGAAGTGATACCATCC TGTAACCATAGAGGAGCCGT 55.0 50.0 58.1 57.8 1900 bp 0.5 57.7
ndhI_p5 CAGCGAAGTGATACCATCCC CTGTGGCGTCAACCTATAGG 55.0 55.0 58.1 57.8 1742 bp 0.5 57.2