Hotspot

atpF

LSC · 10194-11687 · 1494 bp

Back to Tacca

Pi 0.0356
MarkerSeek score 68.10
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1494
variable_sites
51
pi
0.0356
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 CCGCCATCACTTCATCAAGA AAGCCGTAGAAGGTATTGCG 50.0 50.0 58.0 58.1 2233-2288 bp 1.0 85.0
atpF_p2 CACTAATTCACCCGCCATCA AAGCCGTAGAAGGTATTGCG 50.0 50.0 58.0 58.1 2244-2299 bp 1.0 84.9
atpF_p3 ACTAATTCACCCGCCATCAC AAGCCGTAGAAGGTATTGCG 50.0 50.0 58.0 58.1 2243-2298 bp 1.0 84.9
atpF_p4 GCCCTGTTTGAAGAGGTTCA AAGCCGTAGAAGGTATTGCG 50.0 50.0 58.0 58.1 2533-2588 bp 1.0 84.6
atpF_p5 GCCCTGTTTGAAGAGGTTCA GCTCGCTTTCTTCCTTCTCA 50.0 50.0 58.0 57.9 2207-2268 bp 1.0 84.6