Hotspot

rpl32-trnL-UAG

SSC · 121233-121961 · 729 bp

Back to Tacca

Pi 0.0501
MarkerSeek score 77.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
729
variable_sites
35
pi
0.0501
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCGATTTCTACGGGGCATTC AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 794-817 bp 1.0 84.8
rpl32-trnL-UAG_p2 TCGATTTCTACGGGGCATTC CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 795-818 bp 1.0 84.8
rpl32-trnL-UAG_p3 GATTTCTACGGGGCATTCGA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 792 bp 0.5 58.1
rpl32-trnL-UAG_p4 GATTTCTACGGGGCATTCGA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 793 bp 0.5 58.1
rpl32-trnL-UAG_p5 GATTTCTACGGGGCATTCGA TTTATGAGATGCCGCCACTC 50.0 50.0 57.8 58.0 852 bp 0.5 57.8