Hotspot

ndhF-rpl32

SSC · 120359-121085 · 727 bp

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Pi 0.0831
MarkerSeek score 76.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
727
variable_sites
49
pi
0.0831
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGTATAAGAGGAGCTGCA GAATGCCCCGTAGAAATCGA 50.0 50.0 57.8 57.8 949-1064 bp 1.0 85.7
ndhF-rpl32_p2 TGGGTATAAGAGGAGCTGCA TCGAATGCCCCGTAGAAATC 50.0 50.0 57.8 57.8 1066 bp 0.5 56.7
ndhF-rpl32_p3 TGGGTATAAGAGGAGCTGCA TTTCGAATGCCCCGTAGAAA 50.0 45.0 57.8 57.5 1068 bp 0.5 54.8
ndhF-rpl32_p4 TCCTTTCCACTCAAATGCGA GAATGCCCCGTAGAAATCGA 45.0 50.0 57.4 57.8 1339 bp 0.5 53.6
ndhF-rpl32_p5 TCCTTTCCACTCAAATGCGA TCGAATGCCCCGTAGAAATC 45.0 50.0 57.4 57.8 1341 bp 0.5 53.6