Hotspot

trnW-CCA-trnP-UGG

LSC · 66336-66496 · 161 bp

Back to Tacca

Pi 0.0596
MarkerSeek score 77.90
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
161
variable_sites
9
pi
0.0596
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCGTGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 220-230 bp 1.0 85.6
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCGTGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 218-228 bp 1.0 85.6
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCGTGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 221-231 bp 1.0 85.6
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCGTGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 231-241 bp 1.0 85.3
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCGTGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 232-242 bp 1.0 85.3