Hotspot

accD-psaI

LSC · 58888-59374 · 487 bp

Back to Tacca

Pi 0.049
MarkerSeek score 79.70
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
487
variable_sites
23
pi
0.049
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCACGGTTTCCTTCCTTTGA CCATTGCAATTGCCGGAAAT 45.0 45.0 57.0 58.0 583-591 bp 1.0 77.3
accD-psaI_p2 ACTTCACGGTTTCCTTCCTT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.0 58.0 586-594 bp 1.0 77.2
accD-psaI_p3 AACTTCACGGTTTCCTTCCT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.0 58.0 587-595 bp 1.0 77.1
accD-psaI_p4 CAACTTCACGGTTTCCTTCC CCATTGCAATTGCCGGAAAT 50.0 45.0 56.9 58.0 588-596 bp 1.0 76.9
accD-psaI_p5 TCACGGTTTCCTTCCTTTGA AAGAAGCCATTGCAATTGCC 45.0 45.0 57.0 57.9 589-597 bp 1.0 76.6