Hotspot

trnT-GGU-psbD

LSC · 31490-32538 · 1049 bp

Back to Tacca

Pi 0.0418
MarkerSeek score 70.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1049
variable_sites
43
pi
0.0418
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTAACGCCATGGTAAGACGT AAGCACCATGAAGAGCAACA 50.0 45.0 58.0 58.0 1460-1464 bp 1.0 83.7
trnT-GGU-psbD_p2 GTAACGCCATGGTAAGACGT AGCACCATGAAGAGCAACAA 50.0 45.0 58.0 58.0 1459-1463 bp 1.0 83.7
trnT-GGU-psbD_p3 GTAACGCCATGGTAAGACGT ACAAAAGTCCACAGACCACC 50.0 50.0 58.0 57.9 1443-1447 bp 1.0 83.4
trnT-GGU-psbD_p4 GTAACGCCATGGTAAGACGT ATAGCAAAGCAGCGCCTAAT 50.0 45.0 58.0 58.0 1733-1737 bp 1.0 83.1
trnT-GGU-psbD_p5 GTAACGCCATGGTAAGACGT AAAGCAGCGCCTAATACTCC 50.0 50.0 58.0 58.0 1728-1732 bp 1.0 83.0