Hotspot

atpH-atpI

LSC · 12360-13147 · 788 bp

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Pi 0.0429
MarkerSeek score 80.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
788
variable_sites
33
pi
0.0429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACAGAAGCGGCAGAAATCA TTTTGCAACTTTAGCTGCGG 45.0 45.0 58.1 57.9 865-873 bp 1.0 82.8
atpH-atpI_p2 CGCAATACCTTCTACGGCTT TTTTGCAACTTTAGCTGCGG 50.0 45.0 58.1 57.9 955-963 bp 1.0 82.7
atpH-atpI_p3 AACAGAAGCGGCAGAAATCA CGAATCCATGGAGGGTCATC 45.0 55.0 58.1 57.8 835-843 bp 1.0 82.6
atpH-atpI_p4 CGCAATACCTTCTACGGCTT CGAATCCATGGAGGGTCATC 50.0 55.0 58.1 57.8 925-933 bp 1.0 82.5
atpH-atpI_p5 AGCCAATCCAGCAGCAATAA TTTTGCAACTTTAGCTGCGG 45.0 45.0 57.9 57.9 883-891 bp 1.0 82.4