Hotspot

trnS-GCU-trnG-UCC

LSC · 6403-7421 · 1019 bp

Back to Tacca

Pi 0.0536
MarkerSeek score 71.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1019
variable_sites
24
pi
0.0536
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.44
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAGATTAGCAATCCACCGCT ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 546-1096 bp 1.0 76.1
trnS-GCU-trnG-UCC_p2 CAGATTAGCAATCCACCGCT ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 546-1096 bp 1.0 76.1
trnS-GCU-trnG-UCC_p3 CAATCCACCGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 57.9 58.2 538-1088 bp 1.0 75.6
trnS-GCU-trnG-UCC_p4 AATCCACCGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 57.9 58.2 537-1087 bp 1.0 75.6
trnS-GCU-trnG-UCC_p5 CAATCCACCGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 57.9 56.8 538-1088 bp 1.0 75.5