Hotspot

psbK-psbI

LSC · 5723-6141 · 419 bp

Back to Tacca

Pi 0.0638
MarkerSeek score 79.20
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
419
variable_sites
24
pi
0.0638
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TCACCACTGTGTAAACAAAGAGT 50.0 39.1 57.9 58.2 465-486 bp 1.0 62.3
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG CACCACTGTGTAAACAAAGAGT 50.0 40.9 57.9 56.8 464-485 bp 1.0 61.8
psbK-psbI_p3 AATCGTAGACGTTATGCCCG TCACCACTGTGTAAACAAAGAGT 50.0 39.1 57.9 58.2 517-538 bp 1.0 61.3
psbK-psbI_p4 AATCGTAGACGTTATGCCCG CACCACTGTGTAAACAAAGAGT 50.0 40.9 57.9 56.8 516-537 bp 1.0 60.8
psbK-psbI_p5 ATGCCCGTCATACCTGTACT TCACCACTGTGTAAACAAAGAGT 50.0 39.1 58.2 58.2 504-525 bp 1.0 60.8