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psbE-petL

LSC · 72571-73745 · 1175 bp

Back to Tabernaemontana

Pi 0.00598

MarkerSeek score 62.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1175
variable_sites: 7
pi: 0.00598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.9	56.9	1322-1328 bp	1.0	71.2
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.9	56.9	1321-1327 bp	1.0	71.2
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCAAC	50.0	50.0	57.9	56.8	1320-1326 bp	1.0	70.9
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCA	50.0	45.0	57.8	56.9	1245-1251 bp	1.0	70.4
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCGCTAGTAGAAAACCAA	50.0	45.0	57.8	56.9	1244-1250 bp	1.0	70.4