Hotspot

psaA-ycf3

LSC · 48963-49765 · 803 bp

Back to Tabernaemontana

Pi 0.00996

MarkerSeek score 51.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 8
pi: 0.00996
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	909-925 bp	1.0	77.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	903-919 bp	1.0	77.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	ATCACGAGGCGTTTCGAATA	50.0	45.0	57.9	57.4	904-920 bp	1.0	75.2
psaA-ycf3_p4	TTTGGCCCATTCCTCGAAAG	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.5	58.3	913-929 bp	1.0	74.7
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCCG	TGAAGATCACGAGGCGTTTC	50.0	50.0	58.5	58.3	934-950 bp	1.0	74.6