Hotspot

trnD-GUC-trnY-GUA

LSC · 31590-31691 · 102 bp

Back to Tabernaemontana

Pi 0.0294
MarkerSeek score 61.70
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
102
variable_sites
3
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TAGTGGATCTGTCGGGACTG TGGCAATATGTCTACGCTGG 55.0 50.0 58.2 57.8 228 bp 1.0 77.3
trnD-GUC-trnY-GUA_p2 TAGTGGATCTGTCGGGACTG GGCAATATGTCTACGCTGGT 55.0 50.0 58.2 57.8 227 bp 1.0 77.2
trnD-GUC-trnY-GUA_p3 TAGTGGATCTGTCGGGACTG GGGGACGGACTGTAAATTCG 55.0 55.0 58.2 58.4 249 bp 1.0 76.3
trnD-GUC-trnY-GUA_p4 TAGTGGATCTGTCGGGACTG GGGACGGACTGTAAATTCGT 55.0 50.0 58.2 57.6 248 bp 1.0 76.1
trnD-GUC-trnY-GUA_p5 TAGTGGATCTGTCGGGACTG GATTCTTCCTGGGTCGATGC 55.0 55.0 58.2 58.4 282 bp 1.0 75.7