Hotspot

petN-psbM

LSC · 29395-30590 · 1196 bp

Back to Tabernaemontana

Pi 0.00759
MarkerSeek score 61.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1196
variable_sites
9
pi
0.00759
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1282-1292 bp 1.0 72.9
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1280-1290 bp 1.0 72.9
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1278-1288 bp 1.0 72.4
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1276-1286 bp 1.0 72.4
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.9 58.0 1279-1289 bp 1.0 72.3